These characters can be unlocked by collecting XP points:
|The titular hero. He wears an all black suit with blue highlights and features an aerodynamic helmet.||A man dressed as a pink unicorn.||A Hero with a fire motif and a tiki head. Likely inspired by Hawaiian mythology.||A hero who wears a red outfit with a golden star on his round helmet. Designed to be reminiscent of the USSR in his design.|
|The Falcon||Buckshot||Gil||Maneko Neko|
|He is said to be SpeedRunner's enemy. He is a man with glasses who wears a white bird costume based off of a chicken.||A deer with a red vest and a rifle. Named after shotgun shells.||A undercover detective wearing a costume basically based from a Shark.||A cat with a red scarf and a golden bell. Based on the Maneki Neko of Japanese culture.|
|The first female character to be added in the game. Has a silver, futuristic outfit and red hair.||A skeleton wearing brown clothing and holds a shovel.||A black cat dressed as a witch.||The Scout from "Team Fortress 2".|
|Luc J'adore||Sherlock Bones||Jailbird||Excel/XL|
|A macho guy with a blue mask and rose.||A dog with bright yellow colour, wears Sherlock Holmes' outfit.||A female prisoner, she has her hands bound together which make her animations unique.||Excel is a typical business man who carries a suitcase around all the time. XL wears sunglasses, a cap, jacket and jeans, always carries a boombox around. Excel is playable during weekdays, but is replaced by XL on weekends.|
|Burger||The Original Mr. Quick||Flamenco||Dr. Smart|
|A man dressed like a burger, he is overweight and likes to eat a lot of ketchup and mayonnaise.||Looks like he was Speedrunners' predecessor and got replaced by him at some point, looks like a typical old school superhero.||Man dressed like a bull, he runs like a bull too, and he wears a lot of red.||A gorilla eating a banana, dressed as a doctor.|
|Fort Knight||Veloci-T-Rex||Beach Body SpeedRunner||Summertime Cosmo|
|A 'knight' wearing armor out of cardboard and crates, he is equipped with a wooden sword and wears a bag helmet.||A velociraptor dinosaur. Has a unique moveset, and wears black jeans.||SpeedRunner, but in a summer-themed outfit.||Cosmonaut Comrade, but in a summer-themed outfit.|
These characters can be bought on the Steam store, and cannot be unlocked. They are split into two packs:
Team Falcon (YouTuber Pack 1):
Markiplier - He wears a Black & Red outfit with an "M" in the center. This character also has a Pink Mustache.
PeanutButterGamer - Wears an outfit similar to Toon Link from the "The Legend of Zelda" series.
UberHaxorNova - Wears a grey jacket and has a short, pink hairdo.
Jesse Cox - Wears an orange and purple costume with wings.
Team SpeedRunners (YouTuber Pack 2):
PewDiePie - Wears a crown, headphones and a pink outfit.
Strippin - Wears a tiara and a princess costume.
CinnamonToastKen - Wears a bear headdress. Doesn't have an upper costume.
Dodger - Wears a costume based from a fox.
Showtime - Wears a sunglasses and a white outfit.
Cannon Fyodor - Wears a eyepatch, a helmet and a white shirt.
Diamond Diva - Wears a diva costume, her face is makeup in black and white.
Dashwell - Wears a majordome costume.
These characters can be played by typing in their respective commands on the character select screen (case insensitive):
Gang Beast - The character from gang beasts. Command: /gangbeast.
Hoxton - Character from the game PAYDAY 2. Command: /hoxton.
Dallas - Character from the game PAYDAY 2. Command: /dallas.
Wolf - Character from the game PAYDAY 2. Command: /wolf.
Cloaker - Special Enemy from the game PAYDAY 2. Command: /cloaker.
Octodad - The main character in Octodad: Dadliest Catch. Command: /octodad.
These characters were removed in the r44.9 patch (6 October 2015):
Potato - Normal potato. Command: /sallad ( not accessible )
Goat - The goat from "Goat Simulator". Command: /baaaahhh!!! (not accessible)
QWOP - The character from "QWOP" the game. Command: /qwop (not accessible)
PewDuckPie - A duck in a barrel. Command: /pewduckpie (was only accessible by certain people) .